prime script rt master mix kit (TaKaRa)

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TaKaRa prime script rt master mix kit
Prime Script Rt Master Mix Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 27062 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prime+script+rt+master+mix+kit/PrimeScript+RT+Master+Mix/10__1038_slash_s41598___026___69930___4-68-5-12
Average 99 stars, based on 27062 article reviews

prime script rt master mix kit - by Bioz Stars, 2026-10

99/100 stars

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Synthesized:

Article Title: circDLGAP4 ameliorates trophoblastic oxidative stress in HTR8/SVneo trophoblast-derived cells during preeclampsia by enhancing PDK4-driven glycolysis via sponging miR-29c-3p
Article Snippet: .. cDNA was synthesized using the Prime Script RT Master Mix kit from Takara Bio, Japan (Cat# RR036A) for circular RNA and mRNA detection. .. The TaqMan Advanced miRNA cDNA Synthesis Kit from ThermoFisher, USA (Cat# A28007) was utilized for miRNA cDNA synthesis according to the manufacturer’s protocols.

other:

Article Title: Activated microglial exosomes enhance α-Synuclein internalization and accelerate neurodegeneration in lewy body dementia.
Article Snippet: Total RNA was isolated from cells using TRIzol reagent (Invitrogen, USA) according to the manufacturer’s instructions.

Real-time Polymerase Chain Reaction:

Article Title: Peritumoural adipose tissue drives immune evasion in colorectal cancer via adipose–mesenchymal transformation
Article Snippet: TRIzol Reagent (Thermo Fisher Scientific) or RNA Quick Purification Kit (ESscience) was used to extract RNA from tissues and cells according to the manufacturer’s instructions. .. Next, Prime Script RT Master Mix kit (Takara) was used to synthesize complementary DNA, which then served as a template for real-time PCR using the GoTaq qPCR Master Mix kit (Promega) according to the manufacturer’s instructions. β-Actin or β-tubulin was used as the internal control genes, and relative changes in gene expression were analysed using the 2 −Δ Ct or 2 −ΔΔ Ct formula. ..

Article Title: Baicalin protects against myocardial fibrosis through inhibition of DOT1L/COL-1 pathway during diabetic cardiomyopathy.
Article Snippet: These sections were examined using a bright-field microscope (DP71, OLYMPUS). .. Quantitative real-time polymerase chain reaction (RT-qPCR) RNA was extracted from cells using TRIzol reagent (Beyotime,Shanghai,China). cDNA was prepared using a Prime Script RT Master Mix Kit (Takara, Tokyo, Japan). .. RT-qPCR assays were performed using the SYBR Premix Ex TaqTM Kit (Takara, Tokyo, Japan).

Article Title: Peritumoural adipose tissue drives immune evasion in colorectal cancer via adipose-mesenchymal transformation.
Article Snippet: Although peritumoural visceral adipose tissue (tVAT) is anatomically close to tumours such as colorectal cancer, the immune landscape of this tissue and its functional contribution to tumour immunity remain poorly defined.. Here, we performed single-cell RNA analysis on the tVAT from patients with colorectal cancer to map its immune landscape and observed that tVAT exhibited a highly immune-infiltrated microenvironment enriched with lymphocytes, especially tumour-specific CD8+ T cells.. Mechanistically, tVAT competes with the tumour for these immunocytes by activating the CXCL12–CXCR4 axis to promote tumour immune escape.

Control:

Gene Expression:

DNA Amplification:

Article Title: Endothelial cell-derived exosomes inhibit high glucose-induced osteoblast ferroptosis by activating microRNA-335-3p/prostaglandin endoperoxide synthase 2
Article Snippet: Total RNA was isolated from cellular samples or Exos utilizing TRI Reagent (Biosharp, Hefei, China), followed by quantification and assessment of RNA quality via spectrophotometry. .. Subsequently, 2 μg extracted total RNA was used to synthesize high-quality complementary DNA with amplification conducted using the Prime Script RT Master Mix Kit (TaKaRa, Shiga, Japan). .. For reverse transcription-quantitative PCR, the SYBR Green RT-PCR Kit (Biosharp, Hefei, China) was utilized.

Reverse Transcription:

Article Title: Formation of the pre-metastatic niche by COL9A1 + cancer-associated fibroblasts via SDC4 engagement promotes multi-organ metastasis in gastric cancer.
Article Snippet: Background Metastatic dissemination represents the primary cause of mortality in gastric cancer (GC), with multiorgan involvement posing a formidable therapeutic challenge.. While organ-specific adaptations are well-studied, the conserved cellular programs that confer metastatic competence within primary tumors before dissemination remain poorly defined.. Methods We performed integrated single-cell and spatial transcriptomic profiling on paired primary GC tumors and multi-organ metastases.

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